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Immuno Histo Chemistry Reagents
Elisa, Ria, Flow, Fact, IHC and WB antibodies
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the lysosome contains enzymes
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Happy or healthy? How members of the public prioritise farm animal health and natural behaviours
HAPPY Dyes as Light Amplification Media in Thin Films
Comparison of liquid chromatography-tandem mass spectrometry, RIA, and ELISA methods for measurement of urinary estrogens.
Long-term prevention of renal insufficiency, excess matrix gene expression, and glomerular mesangial matrix expansion by treatment with monoclonal antitransforming growth factor-beta antibody in db/db diabetic mice.
MiRNA-494 enhances M1 macrophage polarization via Nrdp1 in ICH mice model.
Troubleshooting In Immunoprecipitation (Ip)
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Detection fluorescence channels of different Real-Time PCR systems
Real-Time PCR
THERMOCYCLER
System Detection
channels
Savvygen probes
channels Remarks
Roche LightCycler® 96 or
LightCycler®480II
465/510 FAM
Color Compensation is
required only for LC480
system
533/580 HEX
533/610 ROX
618/660 Cy5
Applied Biosystems
ABI 7500 fast
FAM FAM
Passive reference
option ROX is not mark
VIC HEX
ROX ROX
Cy5 Cy5
Bio-Rad CFX96 TM
FAM FAM
HEX HEX
ROX ROX
Cy5 Cy5
Agilent AriaMx
FAM FAM
HEX HEX
ROX ROX
Cy5 Cy5
DNA-Technology
DTlite / DTprime
FAM FAM
HEX HEX
ROX ROX
Cy5 Cy5
Smartcycler®
Cepheid
Channel 1 FAM
Channel 2 HEX
Channel 3 ROX
Channel 4 Cy5
Abbott m2000rt
FAM FAM
HEX HEX
ROX ROX
Cy5 Cy5
Rotor-Gene®Q
Qiagen
Green FAM
Yellow HEX
Orange ROX
Red Cy5