Immuno Histo Chemistry Reagents

Pre-clinical demonstration of porcine islet graft function is necessary to support the clinical transplantation of pig islets. C-peptide concentration is an especially useful marker of insulin secretion, because its measurement is not confounded by the presence of exogenous insulin. To measure porcine C-peptide (PCP), researchers in the field exclusively used the Millipore (previously Linco Research) radioimmunoassay (RIA) until 2011, when Mercodia released an alternative enzyme-linked immunosorbent assay (ELISA).

Human ADAM Metallopeptidase Domain 22 (ADAM22) ELISA Kit
abx571072-96tests	Abbexa	96 tests	EUR 786

Human ADAM Metallopeptidase Domain 22 (ADAM22) ELISA Kit
20-abx150547	Abbexa	EUR 7378.00 EUR 3933.00 EUR 911.00	10 × 96 tests 5 × 96 tests 96 tests

Mouse pre-microRNA Expression Construct mir-22
MMIR-22-PA-1	SBI	Bacterial Streak	EUR 684

HGH (Human chorionic gonadotropin) ELISA test
22	Biobase	96T/Box	Ask for price
Description: ELISA based test for quantitative detection of HGH (Human chorionic gonadotropin)

TACE (ADAM-17)
5-01998	CHI Scientific	4 x 1mg	Ask for price

ADAM-17 Antibody
39313-100ul	SAB	100ul	EUR 390

ADAM 17 Antibody
AF6361	Affbiotech	200ul	EUR 304
Description: ADAM 17 Antibody detects endogenous levels of ADAM 17 .

ADAM 17 Antibody
ABF6361	Lifescience Market	100 ug	EUR 438

Sheep ADAM ELISA Kit
ESA0733	Abclonal	96Tests	EUR 521

Mouse ADAM ELISA Kit
EMA0733	Abclonal	96Tests	EUR 521

Monkey ADAM ELISA Kit
EMKA0733	Abclonal	96Tests	EUR 521

Porcine ADAM ELISA Kit
EPA0733	Abclonal	96Tests	EUR 521

Rat ADAM ELISA Kit
ERA0733	Abclonal	96Tests	EUR 521

Rabbit ADAM ELISA Kit
ERTA0733	Abclonal	96Tests	EUR 521

Goat ADAM ELISA Kit
EGTA0733	Abclonal	96Tests	EUR 521

Canine ADAM ELISA Kit
ECA0733	Abclonal	96Tests	EUR 521

Chicken ADAM ELISA Kit
ECKA0733	Abclonal	96Tests	EUR 521

Human ADAM ELISA Kit
EHA0733	Abclonal	96Tests	EUR 521

Bovine ADAM ELISA Kit
EBA0733	Abclonal	96Tests	EUR 521

ADAM 17 (pT735) Antibody
abx011977-100ug	Abbexa	100 ug	EUR 439

ADAM 17 (Thr735) Antibody
12033-100ul	SAB	100ul	EUR 252

ADAM 17 (Thr735) Antibody
12033-50ul	SAB	50ul	EUR 187

ADAM 17 Blocking Peptide
AF6361-BP	Affbiotech	1mg	EUR 195

PNPP Substrate
F008-22	Cygnus Technologies	22 ml	EUR 180
Description: PNPP Substrate by Cygnus Technologies is available in Europe via Gentaur.

Anti-Dimethyl Histone H3 (Lys23) Rabbit Monoclonal Antibody, Clone#RM171
M06819-22	BosterBio	100ug	EUR 468
Description: Anti-Dimethyl Histone H3 (Lys23) Rabbit Monoclonal Antibody, Clone#RM171 tested in WB, ELISA, Multiplex, reactive to All Vertebrates

IL-11, Interleukin-11, human
RC212-22	Bio Basic	2ug	EUR 104.38

EMAP-II, Endothelial-Monocyte Activating Polypeptide II, human
RC214-22	Bio Basic	5ug	EUR 104.38

Amphiregulin (AREG), human
RC216-22	Bio Basic	50ug	EUR 152.66

ErbB3, ErbB3 Fragment, human
RC218-22	Bio Basic	5ug	EUR 101.33

IL-11, Interleukin-11, murine (mouse)
RC232-22	Bio Basic	2ug	EUR 104.38

I-TAC, CXCL11, human
RC312-22	Bio Basic	5ug	EUR 104.38

Eotaxin, CCL11, human
RC315-22	Bio Basic	5ug	EUR 104.38

Eotaxin, CCL11, murine (mouse)
RC335-22	Bio Basic	5ug	EUR 104.38

CCL11, rat
RC355-22	Bio Basic	5ug	EUR 101.33

Colon Tissue Lysate (Normal)
1715-22	ProSci	0.1 mg	EUR 217.25
Description: Colon tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Claudin 22 (Claudin 22) Antibody
abx231736-100ug	Abbexa	100 ug	EUR 481

IL-22, Interleukin-22, human
RC212-33	Bio Basic	2ug	EUR 104.38

IL-22, Interleukin-22, rat
RC252-33	Bio Basic	2ug	EUR 104.38

Guinea Pig ADAM ELISA Kit
EGA0733	Abclonal	96Tests	EUR 521

ADAM 17 (Cleaved-Arg215) Antibody
20-abx015548	Abbexa	EUR 314.00 EUR 98.00 EUR 398.00 EUR 495.00	100 ug 10 ug 200 ug 300 µg

Phospho-ADAM 17 (Thr735) Antibody
AF3361	Affbiotech	200ul	EUR 304
Description: Phospho-ADAM 17 (Thr735) Antibody detects endogenous levels of ADAM 17 only when phosphorylated at Threonine 735.

Phospho- ADAM 17 (Thr735) Antibody
ABF3361	Lifescience Market	100 ug	EUR 438

Recombinant Human Interleukin-22/IL-22
CH50-10ug	Novoprotein	10ug	EUR 202
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.

Recombinant Human Interleukin-22/IL-22
CH50-1mg	Novoprotein	1mg	EUR 2486
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.

Recombinant Human Interleukin-22/IL-22
CH50-500ug	Novoprotein	500ug	EUR 1613
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.

Recombinant Human Interleukin-22/IL-22
CH50-50ug	Novoprotein	50ug	EUR 496
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.

Recombinant Mouse Interleukin-22/IL-22
CM72-10ug	Novoprotein	10ug	EUR 202
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Recombinant Mouse Interleukin-22/IL-22
CM72-1mg	Novoprotein	1mg	EUR 2486
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Recombinant Mouse Interleukin-22/IL-22
CM72-500ug	Novoprotein	500ug	EUR 1755
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Recombinant Mouse Interleukin-22/IL-22
CM72-50ug	Novoprotein	50ug	EUR 496
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Flagelin 22
HY-P1568	MedChemExpress	10mg	EUR 751

NKL 22
HY-100384	MedChemExpress	10mM/1mL	EUR 126

TTP 22
HY-15479	MedChemExpress	10mg	EUR 201

IL-22
E21-047	EnoGene	10ug	EUR 343

MM-22
B5527-10	ApexBio	10 mg	EUR 350

MM-22
B5527-50	ApexBio	50 mg	EUR 1314

Decynium 22
B5694-50	ApexBio	50 mg	EUR 161

TTP 22
A3894-10	ApexBio	10 mg	EUR 168
Description: IC50= 0.1 ?M; Ki= 40 nMTTP 22 is a high affinity, ATP-competitive casein kinase 2 (CK2) inhibitor.

TTP 22
A3894-5.1	ApexBio	10 mM (in 1mL DMSO)	EUR 190
Description: IC50= 0.1 ?M; Ki= 40 nMTTP 22 is a high affinity, ATP-competitive casein kinase 2 (CK2) inhibitor.

TTP 22
A3894-50	ApexBio	50 mg	EUR 586
Description: IC50= 0.1 ?M; Ki= 40 nMTTP 22 is a high affinity, ATP-competitive casein kinase 2 (CK2) inhibitor.

FGF-22
PR27021	Neuromics	2 ug	EUR 191

TTP-22
B2565-1	Biovision		EUR 131

TTP-22
B2565-5	Biovision		EUR 349

22-Dehydroclerosterol
TB00451	ChemNorm	5mg	EUR 634

ADAM Metallopeptidase Domain 12 (ADAM12) Antibody
20-abx302084	Abbexa	EUR 411.00 EUR 1845.00 EUR 599.00 EUR 182.00 EUR 300.00	100 ug 1 mg 200 ug 20 ug 50 ug

ADAM Like Decysin 1 (ADAMDEC1) Antibody
20-abx303001	Abbexa	EUR 411.00 EUR 1845.00 EUR 599.00 EUR 182.00 EUR 300.00	100 ug 1 mg 200 ug 20 ug 50 ug

ADAM Metallopeptidase Domain 10 (ADAM10) Antibody
abx147972-100ug	Abbexa	100 ug	EUR 439

ADAM Metallopeptidase Domain 8 (ADAM8) Antibody
20-abx147984	Abbexa	EUR 425.00 EUR 342.00	100 ug 50 ug

ADAM Metallopeptidase Domain 12 (ADAM12) Antibody
20-abx121006	Abbexa	EUR 300.00 EUR 439.00 EUR 189.00	100 ul 200 ul 30 ul

ADAM Metallopeptidase Domain 19 (ADAM19) Antibody
20-abx121007	Abbexa	EUR 300.00 EUR 439.00 EUR 189.00	100 ul 200 ul 30 ul

ADAM Metallopeptidase Domain 28 (ADAM28) Antibody
20-abx121252	Abbexa	EUR 300.00 EUR 439.00 EUR 189.00	100 ul 200 ul 30 ul

ADAM Like Decysin 1 (ADAMDEC1) Antibody
20-abx121253	Abbexa	EUR 300.00 EUR 439.00 EUR 189.00	100 ul 200 ul 30 ul

ADAM Metallopeptidase Domain 10 (ADAM10) Antibody
20-abx125489	Abbexa	EUR 411.00 EUR 592.00 EUR 182.00 EUR 314.00	100 ul 200 ul 20 ul 50 ul

ADAM Metallopeptidase Domain 19 (ADAM19) Antibody
20-abx125490	Abbexa	EUR 411.00 EUR 592.00 EUR 182.00 EUR 314.00	100 ul 200 ul 20 ul 50 ul

ADAM Metallopeptidase Domain 8 (ADAM8) Antibody
20-abx125491	Abbexa	EUR 411.00 EUR 592.00 EUR 182.00 EUR 314.00	100 ul 200 ul 20 ul 50 ul

ADAM Metallopeptidase Domain 2 (ADAM2) Antibody
20-abx136084	Abbexa	EUR 411.00 EUR 592.00 EUR 182.00 EUR 314.00	100 ul 200 ul 20 ul 50 ul

ADAM Metallopeptidase Domain 28 (ADAM28) Antibody
20-abx124064	Abbexa	EUR 411.00 EUR 592.00 EUR 182.00 EUR 314.00	100 ul 200 ul 20 ul 50 ul

ADAM Metallopeptidase Domain 8 (ADAM8) Antibody
20-abx124086	Abbexa	EUR 411.00 EUR 592.00	100 ul 200 ul

ADAM Metallopeptidase Domain 29 (ADAM29) Antibody
abx038387-100ug	Abbexa	100 ug	EUR 391

ADAM Metallopeptidase Domain 33 (ADAM33) Antibody
abx038437-100ug	Abbexa	100 ug	EUR 391

ADAM Metallopeptidase Domain 10 (ADAM10) Antibody
abx038455-100ug	Abbexa	100 ug	EUR 391

ADAM Metallopeptidase Domain 2 (ADAM2) Antibody
abx036791-100ug	Abbexa	100 ug	EUR 391

ADAM Like Decysin 1 (ADAMDEC1) Antibody
abx037055-100ug	Abbexa	100 ug	EUR 391

ADAM Metallopeptidase Domain 17 (ADAM17) Antibody
abx037459-100ug	Abbexa	100 ug	EUR 391

ADAM Metallopeptidase Domain 19 (ADAM19) Antibody
abx037566-100ug	Abbexa	100 ug	EUR 391

ADAM Metallopeptidase Domain 19 (ADAM19) Antibody
abx038255-100ug	Abbexa	100 ug	EUR 391

ADAM Metallopeptidase Domain 12 (ADAM12) Antibody
20-abx006584	Abbexa	EUR 411.00 EUR 592.00 EUR 182.00 EUR 314.00	100 ul 200 ul 20 ul 50 ul

ADAM Metallopeptidase Domain 17 (ADAM17) Antibody
20-abx000869	Abbexa	EUR 411.00 EUR 592.00 EUR 182.00 EUR 314.00	100 ul 200 ul 20 ul 50 ul

ADAM Metallopeptidase Domain 9 (ADAM9) Antibody
20-abx004126	Abbexa	EUR 411.00 EUR 592.00 EUR 182.00 EUR 314.00	100 ul 200 ul 20 ul 50 ul

ADAM Metallopeptidase Domain 33 (ADAM33) Antibody
20-abx005774	Abbexa	EUR 411.00 EUR 592.00 EUR 182.00 EUR 314.00	100 ul 200 ul 20 ul 50 ul

ADAM Metallopeptidase Domain 8 (ADAM8) Antibody
abx027773-400ul	Abbexa	400 ul	EUR 523

ADAM Metallopeptidase Domain 8 (ADAM8) Antibody
abx027773-80l	Abbexa	80 µl	EUR 286

ADAM Metallopeptidase Domain 9 (ADAM9) Antibody
abx033395-400ul	Abbexa	400 ul	EUR 523

ADAM Metallopeptidase Domain 9 (ADAM9) Antibody
abx033395-80l	Abbexa	80 µl	EUR 286

ADAM Metallopeptidase Domain 17 (ADAM17) Antibody
abx028423-400ul	Abbexa	400 ul	EUR 523

ADAM Metallopeptidase Domain 17 (ADAM17) Antibody
abx028423-80l	Abbexa	80 µl	EUR 286

(At the end of 2013, the Millipore RIA was withdrawn from the market for commercial reasons.) In our current study, to directly compare these two assays, we performed validation studies on each. We also performed interlaboratory comparisons. Then, to determine the level of agreement between the assays, we analyzed the porcine serum C-peptide concentration measurement results obtained from each assay.