Evaluation of commercial ELISA and RIA for measuring porcine C-peptide: implications for research.

Pre-clinical demonstration of porcine islet graft function is necessary to support the clinical transplantation of pig islets. C-peptide concentration is an especially useful marker of insulin secretion, because its measurement is not confounded by the presence of exogenous insulin. To measure porcine C-peptide (PCP), researchers in the field exclusively used the Millipore (previously Linco Research) radioimmunoassay (RIA) until 2011, when Mercodia released an alternative enzyme-linked immunosorbent assay (ELISA).

Human ADAM Metallopeptidase Domain 22 (ADAM22) ELISA Kit

abx571072-96tests 96 tests
EUR 786

Human ADAM Metallopeptidase Domain 22 (ADAM22) ELISA Kit

20-abx150547
  • EUR 7378.00
  • EUR 3933.00
  • EUR 911.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Mouse pre-microRNA Expression Construct mir-22

MMIR-22-PA-1 Bacterial Streak
EUR 684

HGH (Human chorionic gonadotropin) ELISA test

22 96T/Box Ask for price
Description: ELISA based test for quantitative detection of HGH (Human chorionic gonadotropin)

TACE (ADAM-17)

5-01998 4 x 1mg Ask for price

ADAM-17 Antibody

39313-100ul 100ul
EUR 390

ADAM 17 Antibody

AF6361 200ul
EUR 304
Description: ADAM 17 Antibody detects endogenous levels of ADAM 17 .

ADAM 17 Antibody

ABF6361 100 ug
EUR 438

Sheep ADAM ELISA Kit

ESA0733 96Tests
EUR 521

Mouse ADAM ELISA Kit

EMA0733 96Tests
EUR 521

Monkey ADAM ELISA Kit

EMKA0733 96Tests
EUR 521

Porcine ADAM ELISA Kit

EPA0733 96Tests
EUR 521

Rat ADAM ELISA Kit

ERA0733 96Tests
EUR 521

Rabbit ADAM ELISA Kit

ERTA0733 96Tests
EUR 521

Goat ADAM ELISA Kit

EGTA0733 96Tests
EUR 521

Canine ADAM ELISA Kit

ECA0733 96Tests
EUR 521

Chicken ADAM ELISA Kit

ECKA0733 96Tests
EUR 521

Human ADAM ELISA Kit

EHA0733 96Tests
EUR 521

Bovine ADAM ELISA Kit

EBA0733 96Tests
EUR 521

ADAM 17 (pT735) Antibody

abx011977-100ug 100 ug
EUR 439

ADAM 17 (Thr735) Antibody

12033-100ul 100ul
EUR 252

ADAM 17 (Thr735) Antibody

12033-50ul 50ul
EUR 187

ADAM 17 Blocking Peptide

AF6361-BP 1mg
EUR 195

PNPP Substrate

F008-22 22 ml
EUR 180
Description: PNPP Substrate by Cygnus Technologies is available in Europe via Gentaur.

Anti-Dimethyl Histone H3 (Lys23) Rabbit Monoclonal Antibody, Clone#RM171

M06819-22 100ug
EUR 468
Description: Anti-Dimethyl Histone H3 (Lys23) Rabbit Monoclonal Antibody, Clone#RM171 tested in WB, ELISA, Multiplex, reactive to All Vertebrates

IL-11, Interleukin-11, human

RC212-22 2ug
EUR 104.38

EMAP-II, Endothelial-Monocyte Activating Polypeptide II, human

RC214-22 5ug
EUR 104.38

Amphiregulin (AREG), human

RC216-22 50ug
EUR 152.66

ErbB3, ErbB3 Fragment, human

RC218-22 5ug
EUR 101.33

IL-11, Interleukin-11, murine (mouse)

RC232-22 2ug
EUR 104.38

I-TAC, CXCL11, human

RC312-22 5ug
EUR 104.38

Eotaxin, CCL11, human

RC315-22 5ug
EUR 104.38

Eotaxin, CCL11, murine (mouse)

RC335-22 5ug
EUR 104.38

CCL11, rat

RC355-22 5ug
EUR 101.33

Colon Tissue Lysate (Normal)

1715-22 0.1 mg
EUR 217.25
Description: Colon tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Claudin 22 (Claudin 22) Antibody

abx231736-100ug 100 ug
EUR 481

IL-22, Interleukin-22, human

RC212-33 2ug
EUR 104.38

IL-22, Interleukin-22, rat

RC252-33 2ug
EUR 104.38

Guinea Pig ADAM ELISA Kit

EGA0733 96Tests
EUR 521

ADAM 17 (Cleaved-Arg215) Antibody

20-abx015548
  • EUR 314.00
  • EUR 98.00
  • EUR 398.00
  • EUR 495.00
  • 100 ug
  • 10 ug
  • 200 ug
  • 300 µg

Phospho-ADAM 17 (Thr735) Antibody

AF3361 200ul
EUR 304
Description: Phospho-ADAM 17 (Thr735) Antibody detects endogenous levels of ADAM 17 only when phosphorylated at Threonine 735.

Phospho- ADAM 17 (Thr735) Antibody

ABF3361 100 ug
EUR 438

Recombinant Human Interleukin-22/IL-22

CH50-10ug 10ug
EUR 202
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.

Recombinant Human Interleukin-22/IL-22

CH50-1mg 1mg
EUR 2486
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.

Recombinant Human Interleukin-22/IL-22

CH50-500ug 500ug
EUR 1613
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.

Recombinant Human Interleukin-22/IL-22

CH50-50ug 50ug
EUR 496
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.

Recombinant Mouse Interleukin-22/IL-22

CM72-10ug 10ug
EUR 202
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Recombinant Mouse Interleukin-22/IL-22

CM72-1mg 1mg
EUR 2486
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Recombinant Mouse Interleukin-22/IL-22

CM72-500ug 500ug
EUR 1755
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Recombinant Mouse Interleukin-22/IL-22

CM72-50ug 50ug
EUR 496
Description: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Flagelin 22

HY-P1568 10mg
EUR 751

NKL 22

HY-100384 10mM/1mL
EUR 126

TTP 22

HY-15479 10mg
EUR 201

IL-22

E21-047 10ug
EUR 343

MM-22

B5527-10 10 mg
EUR 350

MM-22

B5527-50 50 mg
EUR 1314

Decynium 22

B5694-50 50 mg
EUR 161

TTP 22

A3894-10 10 mg
EUR 168
Description: IC50= 0.1 ?M; Ki= 40 nMTTP 22 is a high affinity, ATP-competitive casein kinase 2 (CK2) inhibitor.

TTP 22

A3894-5.1 10 mM (in 1mL DMSO)
EUR 190
Description: IC50= 0.1 ?M; Ki= 40 nMTTP 22 is a high affinity, ATP-competitive casein kinase 2 (CK2) inhibitor.

TTP 22

A3894-50 50 mg
EUR 586
Description: IC50= 0.1 ?M; Ki= 40 nMTTP 22 is a high affinity, ATP-competitive casein kinase 2 (CK2) inhibitor.

FGF-22

PR27021 2 ug
EUR 191

TTP-22

B2565-1
EUR 131

TTP-22

B2565-5
EUR 349

22-Dehydroclerosterol

TB00451 5mg
EUR 634

ADAM Metallopeptidase Domain 12 (ADAM12) Antibody

20-abx302084
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

ADAM Like Decysin 1 (ADAMDEC1) Antibody

20-abx303001
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

ADAM Metallopeptidase Domain 10 (ADAM10) Antibody

abx147972-100ug 100 ug
EUR 439

ADAM Metallopeptidase Domain 8 (ADAM8) Antibody

20-abx147984
  • EUR 425.00
  • EUR 342.00
  • 100 ug
  • 50 ug

ADAM Metallopeptidase Domain 12 (ADAM12) Antibody

20-abx121006
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 100 ul
  • 200 ul
  • 30 ul

ADAM Metallopeptidase Domain 19 (ADAM19) Antibody

20-abx121007
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 100 ul
  • 200 ul
  • 30 ul

ADAM Metallopeptidase Domain 28 (ADAM28) Antibody

20-abx121252
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 100 ul
  • 200 ul
  • 30 ul

ADAM Like Decysin 1 (ADAMDEC1) Antibody

20-abx121253
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 100 ul
  • 200 ul
  • 30 ul

ADAM Metallopeptidase Domain 10 (ADAM10) Antibody

20-abx125489
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

ADAM Metallopeptidase Domain 19 (ADAM19) Antibody

20-abx125490
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

ADAM Metallopeptidase Domain 8 (ADAM8) Antibody

20-abx125491
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

ADAM Metallopeptidase Domain 2 (ADAM2) Antibody

20-abx136084
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

ADAM Metallopeptidase Domain 28 (ADAM28) Antibody

20-abx124064
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

ADAM Metallopeptidase Domain 8 (ADAM8) Antibody

20-abx124086
  • EUR 411.00
  • EUR 592.00
  • 100 ul
  • 200 ul

ADAM Metallopeptidase Domain 29 (ADAM29) Antibody

abx038387-100ug 100 ug
EUR 391

ADAM Metallopeptidase Domain 33 (ADAM33) Antibody

abx038437-100ug 100 ug
EUR 391

ADAM Metallopeptidase Domain 10 (ADAM10) Antibody

abx038455-100ug 100 ug
EUR 391

ADAM Metallopeptidase Domain 2 (ADAM2) Antibody

abx036791-100ug 100 ug
EUR 391

ADAM Like Decysin 1 (ADAMDEC1) Antibody

abx037055-100ug 100 ug
EUR 391

ADAM Metallopeptidase Domain 17 (ADAM17) Antibody

abx037459-100ug 100 ug
EUR 391

ADAM Metallopeptidase Domain 19 (ADAM19) Antibody

abx037566-100ug 100 ug
EUR 391

ADAM Metallopeptidase Domain 19 (ADAM19) Antibody

abx038255-100ug 100 ug
EUR 391

ADAM Metallopeptidase Domain 12 (ADAM12) Antibody

20-abx006584
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

ADAM Metallopeptidase Domain 17 (ADAM17) Antibody

20-abx000869
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

ADAM Metallopeptidase Domain 9 (ADAM9) Antibody

20-abx004126
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

ADAM Metallopeptidase Domain 33 (ADAM33) Antibody

20-abx005774
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

ADAM Metallopeptidase Domain 8 (ADAM8) Antibody

abx027773-400ul 400 ul
EUR 523

ADAM Metallopeptidase Domain 8 (ADAM8) Antibody

abx027773-80l 80 µl
EUR 286

ADAM Metallopeptidase Domain 9 (ADAM9) Antibody

abx033395-400ul 400 ul
EUR 523

ADAM Metallopeptidase Domain 9 (ADAM9) Antibody

abx033395-80l 80 µl
EUR 286

ADAM Metallopeptidase Domain 17 (ADAM17) Antibody

abx028423-400ul 400 ul
EUR 523

ADAM Metallopeptidase Domain 17 (ADAM17) Antibody

abx028423-80l 80 µl
EUR 286

(At the end of 2013, the Millipore RIA was withdrawn from the market for commercial reasons.) In our current study, to directly compare these two assays, we performed validation studies on each. We also performed interlaboratory comparisons. Then, to determine the level of agreement between the assays, we analyzed the porcine serum C-peptide concentration measurement results obtained from each assay.